Diagnostic Techniques Viruses

• Direct Examination

  • Light Microscopy

  • Fluorescent Microscopy

    • Direct FA: Antigen plus patient's serum will produce antibody if patient's serum contains the appropriate specific antibody.

    • Indirect FA: Antigen + antibody + anti-antibodies. It is cheaper and more commonly used.

  • Electron Microscopy

    • Immune Electron Microscopy

  • Solid Phase Immunoassay: Most common method today

    • RIA

    • ELISA

  • Nucleic Acid Hybridization

• Isolation and Identification

• Serodiagnosis

  • RIA: Detection of antigen.

  • ELISA: Detection of antigen. Commonly done on HIV.

  • Neutralization Test: Mix known virus with patient's serum, and if the patient's serum contains antibodies then viral growth will be inhibited.

  • Hemagglutinin Test:

Category: Microbiology Notes