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The lesions often contain few parasites. Diagnosis is sometimes made solely on a clinical basis. Culture of the parasites is possible, but not really feasible in primitive rural conditions. Serology in espundia can be positive or negative (the quality of the antigen is of crucial importance). A practical problem in South America is whether a certain skin lesion with
Leishmania amastigotes is caused by
L. braziliensis or not. The geographical origin of the lesion or PCR and/or zymodeme analyses may give an answer here, though these laboratory techniques are not available in rural areas.
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Medicine Notes
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