Evaluating putative virulence factors: “molecular Koch’s postulates”
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suspected virulence trait should be associated significantly more often with pathogenic strains than with non-pathogenic strains
specific inactivation of genes associated with suspected virulence trait should lead to a measurable decrease in virulence
replacement of inactivated genes with wild-type version should restore pathogenicity
these are straightforward ideas
Category: Microbiology Notes
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