Ziehl-Neelson stain (acid-fast stain)

on 3.9.08 with 0 comments



  • why it works: cell envelope of Mycobacterium is unique because it has a certain type of acid

  • it takes a bunch of chemicals to induce a reaction; this is the acid-fast stain



Nocardia: modified acid-fast test yields a positive result

  • because it has a lot of lipids, it stains poorly with Gram stain, looking like a branching, poorly-staining Gram-positive organism

  • it causes brain and pulmonary abscesses

  • modified acid-fast test with gentler acid decolorizer leads to characteristic picture

Category: Microbiology Notes

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