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1. Morphology and Identification
- Comma-shaped, curved rod with a polar flagellum
- Oxidase-positive
- Rapidly killed by acid & grow at a very high pH (8.5-9.5)
- Halotolerant or halophilic : grows on media containing 6% NaCl
2. Antigenic Structure
- A single heat-labile flagella H antigen
- O lipopolysaccharides : serologic specificity
- V cholerae serogroups O1 and O139; classic cholera (Table 18-1)
- V cholerae serogroups non-O1 and non-O139 ; Cholera-like diarrhea
3. Vibrio cholerae Enterotoxin
Produce a heat-labile enterotoxin (Subunits A and B); chromosomal control (the same mechnism of action as E.coli's LT toxin, but LT is transmitted via s plasmid)
binding to Ganglioside GM1 on intestinal epithelium by subunit B ≫ entry of the A subunit ≫ Subunit A activate G-protein ≫ stimulate the activity of a membrane-bound adenylate cyclase ≫ production intracellular cAMP ≫ secretion of Na and Cl and inhibition of Na and Cl reabsorption ≫ prolonged hypersecretion of water and electrolytes ≫ diarrhea (20-30 L/d) ≫ dehydration
4. Pathogenesis & Clinical Findings
- Infective dose: 108-1010 organisms
- Not an invasive infection
- About 60% of infection with classic V Cholerae: Asymptomatic
- Incubation period (1-4 days) ≫ Sudden onset of nausea and vomiting, profuse diarrhea with abdominal cramps, rice-water like stool (mucus, epithelial cells, large numbers of vibrios) ≫ profound dehydration, circulatory collapse and anuria, mortality rate without treatment is 25-50%
5. Immunity ; Partial immunity to reinfection
- some protection by gastric acid
6. Treatment
- Water and electrolyte replacement & oral tetracycline.
7. Epidemiology, Prevention & Control
- Endemic in India and Southeast Asia
- Spread by person-to-person and by water, food, and flies.
- Control: education and improvement of sanitation, particularly of food and water
- Chemoprophylaxis, and repeated injection of a vaccine: limited protection to heavily exposed persons
Category: Microbiology Notes
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