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a. Can add red cells to check for hemagglutinins
b. 100% specificity (for showing a virus is there, not specific ID), poor sensitivity
c. Without antigen detection (shell vial technique) can only make presumptive ID of virus
2. Antigen detection - can do on cell specimens from patient or on culture (shell vial technique)
3. Immunofluorescence assay - Ab labeled w/ fluorescing compound - use UV microscope
4. ELISA, immunoperoxidase - Ab labeled w/ enzyme that changes substrate color
a. Serology for IgG (four-fold rise of two specimensis diagnostic), IgM - single result is diagnostic
b. DNA probes/PCR
c. Electron microscopy for ID of viral particles - expensive, insensitive
Category: Microbiology Notes
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