Flow Cytometry

• Applications of flow cytometry

  • Population analysis

    • Light scatter

      • Forward angle light scatter (FALS) is proportional to cell size

      • 90 light scatter is proportional to cell granularity and nuclear complexity

    • DNA analysis

      • Euploid = normal DNA content

        • Diploid (2n) is the number of chromosomes

        • If a tumor is euploid, it has a better prognosis (with the exception of ALL, which has a better prognosis if its multiploid)

      • Aneuploid = deviations from multiples of haploid number

      • S-phase: the percentage of cells in the process of increasing their DNA for mitosis (i.e., is an indicator of mitotic activity)

    • Cell markers

  • Cell sorting

• CD = cluster of differentiation

  • CD4 = helper T cell

  • CD8 = T_C

Category: Medical Subject Notes , Pathology Notes